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Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications
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Bioinspired Reactive Interfaces Based on Layered Double Hydroxides-Zn Rich Hydroxyapatite with Antibacterial Activity
Anna Donnadio1, Marzia Bini1, Catia Centracchio1
1Dipartimento di Scienze Farmaceutiche, Università di Perugia, Via del Liceo, 1, 06123 Perugia, Italy.
ACS Biomaterials Science & Engineering
|March 19, 2021
Summary
New biocompatible composites combining layered double hydroxides (LDHs) and hydroxyapatite (HA) show antibacterial and osteoinductive properties. These HA@LDH materials are non-toxic to human osteoblasts, offering potential for titanium-based prostheses.
Area of Science:
- Biomaterials Science
- Materials Chemistry
- Nanotechnology
Background:
- Layered double hydroxides (LDHs) and hydroxyapatite (HA) are promising biomaterials.
- Developing biocompatible materials with antimicrobial and osteoinductive properties is crucial for medical implants.
Purpose of the Study:
- To prepare and characterize novel HA@LDH composites.
- To evaluate the biocompatibility, antibacterial activity, and osteoinductive potential of these composites.
- To assess their suitability for titanium-based prostheses.
Main Methods:
- Synthesis of ZnAl and ZnAlGa layered double hydroxides (LDHs).
- Formation of HA@LDH composites via HA precipitation on LDH surfaces.
- Characterization using X-ray diffraction (XRD), Rietveld refinement, elemental analysis, micro-Raman spectroscopy, and scanning electron microscopy (SEM).
- Assessment of antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa.
- Evaluation of cytotoxicity and proliferation effects on human osteoblasts (hFob 1.19).
Main Results:
- Successful synthesis of HA@LDH composites with a mixed Ca-Zn HA phase.
- HA growth along the a-axis, forming flake crystals, was observed.
- Composites exhibited significant antibacterial activity at non-cytotoxic concentrations.
- Gallium (Ga)-containing LDHs enhanced antibacterial efficacy.
- HA reduced LDH cytotoxicity towards osteoblasts without compromising bone-bonding ability.
Conclusions:
- The developed HA@LDH composites possess desirable biocompatibility, antibacterial, and osteoinductive properties.
- These materials show potential for enhancing the performance of titanium-based prostheses.
- The combination of LDHs and HA offers a versatile platform for advanced biomaterial development.
