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Isolation of Murine Lymph Node Stromal Cells
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Immunomodulatory Scaffolds Derived from Lymph Node Extracellular Matrices.

Yi Sun Choi1, Eunseon Jeong1, Jung Seung Lee1

  • 1Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.

ACS Applied Materials & Interfaces
|March 22, 2021
PubMed
Summary
This summary is machine-generated.

Lymph node extracellular matrix (LNEM) biomaterials effectively modulate macrophage behavior for tissue healing. Three-dimensional LNEM hydrogels enhance anti-inflammatory functions and promote M2 macrophage polarization for regenerative medicine applications.

Keywords:
3D hydrogeldecellularized matriximmunomodulationlymph node extracellular matrixmacrophage polarizationmuscle regeneration

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Area of Science:

  • Biomaterials Science
  • Immunology
  • Regenerative Medicine

Background:

  • Macrophage immunomodulation within the tissue microenvironment is crucial for tissue repair.
  • Existing platforms like collagen lack the complexity to fully replicate native microenvironments.
  • Developing biomaterials that mimic native immune niches is essential for advancing regenerative therapies.

Purpose of the Study:

  • To investigate the immunomodulatory potential of lymph node extracellular matrix (LNEM) biomaterials.
  • To compare the efficacy of 2D and 3D LNEM formats in modulating macrophage phenotypes.
  • To evaluate LNEM's utility in tissue modeling and regenerative medicine.

Main Methods:

  • Decellularization of lymph node tissues to create LNEM.
  • Fabrication of 2D LNEM coatings and 3D LNEM hydrogels.
  • Co-culture of macrophages with LNEM constructs and assessment of M2 polarization markers.
  • Testing LNEM in liver organoid drug toxicity models and a mouse volumetric muscle loss model.

Main Results:

  • LNEM demonstrated superior immunomodulatory effects compared to collagen platforms.
  • 3D LNEM hydrogels significantly induced M2 macrophage polarization, enhanced cytokine secretion, and improved phagocytosis.
  • LNEM-conditioned macrophages reduced liver organoid susceptibility to hepatotoxic drugs.
  • In vivo implantation of 3D LNEM promoted macrophage recruitment and M2 polarization, facilitating muscle tissue healing.

Conclusions:

  • Three-dimensional LNEM hydrogels effectively mimic lymph node microenvironments for macrophage immunomodulation.
  • LNEM shows promise as a biomaterial for tissue modeling and regenerative medicine, particularly for enhancing pro-healing macrophage functions.
  • These immune system-mimicking biomaterials offer a platform for developing advanced regenerative therapies and in vivo-like drug testing systems.