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Updated: Nov 12, 2025

DNA-based Fish Species Identification Protocol

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

DNA-based techniques for seafood species authentication.

Anthony J Silva¹, Rosalee S Hellberg¹

¹Chapman University, Schmid College of Science and Technology, Food Science Program, One University Drive, Orange, CA, United States.

Advances in Food and Nutrition Research

|March 22, 2021

Summary

This summary is machine-generated.

DNA testing effectively detects seafood fraud, specifically species substitution, safeguarding consumers and the industry. Various DNA methods offer solutions for authenticating fish and shellfish globally.

Keywords:

Authentication DNA-based methods Fish Food fraud Mislabeling PCR Seafood Sequencing Shellfish Substitution

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DNA-based Fish Species Identification Protocol

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Published on: April 28, 2010

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

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Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

Area of Science:

Food Science
Genetics
Forensic Science

Background:

Global seafood trade expansion has amplified concerns regarding seafood fraud, including species substitution.
Seafood fraud poses significant economic, environmental, and public health risks worldwide.
DNA-based authentication is crucial for regulatory agencies and food fraud risk mitigation.

Purpose of the Study:

To review DNA-based methods for detecting seafood species substitution.
To analyze established and emerging techniques for seafood authentication.
To provide an overview of DNA-based species identification in seafood.

Main Methods:

Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP)
Species-specific PCR and real-time PCR
Sanger sequencing, microarrays, and High-Resolution Melting (HRM)
Emerging techniques: droplet digital PCR, isothermal amplification, PCR-ELISA, and high-throughput sequencing

Main Results:

Multiple DNA-based methods exist for seafood species identification.
Some methods target specific species, while others allow broad differentiation.
Established and novel techniques offer various levels of specificity and throughput.

Conclusions:

DNA-based methods are vital tools for combating seafood fraud and ensuring species authenticity.
A range of techniques, from targeted PCR to high-throughput sequencing, are available for seafood authentication.
Continued development of DNA-based methods is essential for global seafood safety and integrity.