Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Frozen phase separation-fabricated bacterial nanocellulose/polyvinyl alcohol dual-network hydrogel membranes with enhanced mechanical properties and anti-calcification for artificial heart valves.

International journal of biological macromolecules·2026
Same author

Balancing mechanical property and swelling behavior of bacterial cellulose film by in-situ adding chitosan oligosaccharide and covalent crosslinking with γ-PGA.

International journal of biological macromolecules·2024
Same author

β-Alanine enhancing the crosslink of chitosan/poly-(γ-glutamic acid) hydrogel for a potential alkaline-adapted wound dressing.

International journal of biological macromolecules·2023
Same author

GpemDB: A Scalable Database Architecture with the Multi-omics Entity-relationship Model to Integrate Heterogeneous Big-data for Precise Crop Breeding.

Frontiers in bioscience (Landmark edition)·2022
Same author

<i>Dendrobium officinale</i> Enzyme Changing the Structure and Behaviors of Chitosan/γ-poly(glutamic acid) Hydrogel for Potential Skin Care.

Polymers·2022
Same author

Silencing Nogo-B improves the integrity of blood-retinal barrier in diabetic retinopathy via regulating Src, PI3K/Akt and ERK pathways.

Biochemical and biophysical research communications·2021

Related Experiment Video

Updated: Nov 12, 2025

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores
11:38

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Published on: April 5, 2022

2.8K

A rapid and rapid method to quantify poly (γ-glutamic acid) content via copper ion complexation.

Chenrui Yu1, Yipeng Zang1, Li Wang1

  • 1College of Biological and Food Engineering, Anhui Polytechnic University, 241000 Wuhu, China.

International Journal of Biological Macromolecules
|March 22, 2021
PubMed
Summary

A new method using copper ions accurately quantifies poly (γ-glutamic acid) (γ-PGA) in complex samples. This technique offers higher precision and accuracy than existing methods for determining γ-PGA content.

Keywords:
ComplexationCopper ionUltraviolet spectrophotometryWeighingγ-PGA

More Related Videos

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution
07:00

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution

Published on: March 20, 2019

15.5K
Author Spotlight: Innovating Thiol Quantification and Biomarker Detection for Oxidative Stress Research
03:35

Author Spotlight: Innovating Thiol Quantification and Biomarker Detection for Oxidative Stress Research

Published on: June 28, 2024

1.8K

Related Experiment Videos

Last Updated: Nov 12, 2025

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores
11:38

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Published on: April 5, 2022

2.8K
Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution
07:00

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution

Published on: March 20, 2019

15.5K
Author Spotlight: Innovating Thiol Quantification and Biomarker Detection for Oxidative Stress Research
03:35

Author Spotlight: Innovating Thiol Quantification and Biomarker Detection for Oxidative Stress Research

Published on: June 28, 2024

1.8K

Area of Science:

  • Biochemistry
  • Analytical Chemistry

Background:

  • Quantifying poly (γ-glutamic acid) (γ-PGA) is challenging due to impurities in biological samples.
  • Existing methods for γ-PGA determination have limitations in speed and accuracy.

Purpose of the Study:

  • To develop a rapid and accurate method for quantifying γ-PGA content.
  • To overcome the limitations of current γ-PGA determination techniques.

Main Methods:

  • Utilized the complexation reaction between γ-PGA, common impurities (glucose, glutamic acid, proteins), and copper ions.
  • Observed that only γ-PGA forms a precipitate with copper ions, allowing for quantitative analysis.
  • Compared the accuracy and precision of the copper ion method with UV and weighing methods.

Main Results:

  • The copper ion complexation method demonstrated a linear correlation between γ-PGA content and precipitate amount.
  • Achieved high accuracy (95.82%) and precision (99.29%) with the copper ion method.
  • The developed method significantly outperformed UV and weighing techniques in accuracy and precision.

Conclusions:

  • The copper ion complexation method provides a robust and reliable approach for determining γ-PGA content.
  • This method is suitable for quantifying γ-PGA in crude biological samples, offering a significant improvement over existing techniques.
  • The simplicity and accuracy of this method suggest its widespread adoption for γ-PGA analysis.