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Related Experiment Video

Updated: Nov 12, 2025

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

A practical, low-cost, short-term storage method for genomic DNA.

Demir Özdemir¹

¹Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University, 07070 Antalya, Turkey.

|March 22, 2021

Summary

Cellulose paper towel and blotting paper effectively preserve DNA for 130 days at room temperature. These low-cost materials offer reliable DNA preservation without degradation, serving as viable alternatives to commercial products.

Keywords:

DNA preservation FTA cellulose dried blood spots paper towel

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DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

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Published on: March 26, 2011

Area of Science:

Molecular Biology
Forensic Science
Biotechnology

Background:

DNA preservation is crucial for various applications, including diagnostics and forensics.
Commercial DNA preservation products can be expensive.
Low-cost alternatives for DNA storage are needed.

Purpose of the Study:

To evaluate the DNA preservation capabilities of cellulose paper towel and blotting paper.
To compare these materials against commercial DNA preservation products.
To determine the effectiveness of these materials over 130 days at room temperature.

Main Methods:

Chicken blood was used as the DNA source.
Blood spots were applied to paper towel, blotting paper, FTA® cards, and DNA/RNA Shield™.
Samples were stored at room temperature for 130 days.
DNA extraction was performed using Tris-EDTA and NaOH protocols.
PCR activity and DNA yield were assessed.

Main Results:

Paper towel and blotting paper demonstrated significant DNA binding and preservation capacity.
Reliable DNA extraction and PCR activity were observed from samples stored on paper towel.
No DNA degradation was detected after 130 days of storage at room temperature.

Conclusions:

Cellulose-based blotting paper and paper towel are effective and low-cost materials for DNA preservation.
These materials can maintain DNA integrity for at least 130 days at room temperature.
Paper towel and blotting paper are suitable alternatives to commercial DNA preservation solutions.