Separation and Enrichment of Sudan III Using Surface Modified Hollow Glass Microspheres and Colorimetric Detection

Sudan III has been shown to be carcinogenic to human beings due to the azo chemical structure. A simple, highly selective, and environmentally friendly pretreatment method is usually required before the analysis of Sudan III in complex practical samples due to low concentration and matrix interference.

The aim of this research was to prepare buoyant adsorbents, octyl trimethoxysilane caped hollow glass microspheres (HGMs), and establish a new pretreatment method for the detection of Sudan III in real samples.

HGMs were activated and transferred to a flask containing 80 mL ethanol solution (9:1, v/v) and 0.9 mL ammonia. The octyl trimethoxysilane was added to the slurry and covalently coupled on the surface of the HGMs. The modified HGMs were used as adsorbents for the enrichment of Sudan III. After adsorption and desorption, the UV-Vis absorption spectrum was recorded under excitation at 506 nm.

Under the optimum conditions, the linear range and detection limit were 0.10-4.0 mg/L and 0.048 mg/L, respectively. The proposed method was successfully employed to detect Sudan III in chili products with acceptable recoveries of spikes (90.7-102%).

The adsorbent, which could be separated by flotation, provided a new solid phase extraction method for the pretreatment of complex samples.

A new solid phase extraction method was provided for the pretreatment of complex samples. In addition, the adsorbents with high enrichment efficiency can be easily separated by flotation and repeatedly used for separation and enrichment of Sudan III.