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Updated: Nov 12, 2025

Optimization of Processing of Tiebangchui with Highland Barley Wine Based on the Box-Behnken Design Combined with the Entropy Method
Published on: May 19, 2023
Box-Behnken Design-Desirability Function Approach in Optimization of HPLC Method for Simultaneous Determination of
Şule Dinç-Zor1, Özlem Aksu Dönmez1
1Yildiz Technical University, Department of Chemistry, Davutpasa Campus, Istanbul , Turkey, 34220.
Background:
The advantages of simultaneous analyses are a decrease in analysis time and a more economical use of solvents and reagents. It is desirable that HPLC analyses possess both short term and good resolution.
Objective:
The aim of the current study is to develop an HPLC method for the simultaneous determination of ibuprofen, sodium benzoate, methyl paraben and propyl paraben as preservatives, and sunset yellow as a colorant, in syrup formulation.
Method:
To optimize chromatographic separation conditions, multi-response optimization using the Derringer's desirability function was employed for the development of a rapid and efficient HPLC method. The ranges of independent variables used for the optimization process were 50-60% (v/v) for acetonitrile, 5.0-7.0 for pH, and 1.0-2.0 mL/min for flow rate of the mobile phase. The effects of these variables on the output responses, such as critical resolution between sunset yellow and sodium benzoate and retention time of the last peak indicating analysis time of the method, were evaluated by statistical experimental design.
Results:
Optimum conditions fixed for the simultaneous analyses were acetonitrile:phosphate buffer (60:40, v/v), pH 5.0, and a flow rate of 1.8 mL/min. The eluate was monitored using a photo diode detector set at 220 nm. Total chromatographic analysis time was approximately 3 min.
Conclusions:
The developed method validated as per International Conference on Harmonization guidelines was successfully applied for the determination of five compounds in their pharmaceutical formulation.
Highlights:
This efficient method has isocratic elution system and can be used for routine analyses of these compounds in similar pharmaceutical products.
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