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Updated: Nov 11, 2025

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
Development of comprehensive liquid chromatography with diode array and mass spectrometric detection for the
Nagihan Karaaslan Ayhan1, Erwin Rosenberg2
1Department of Chemistry and Chemical Processing Technologies, Tunceli Vocational School, Munzur University, TR 62000 Tunceli, Turkey; Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9/164 AC, Vienna, Austria.
Abstract:
The main objective of the present work was to develop a method for the simultaneous and comprehensive analysis of (poly-)phenolic and flavonoid compounds with liquid chromatography with diode array and mass spectrometric detection and its application to green asparagus samples. To this end, a representative set of polyphenols was used to develop the method. A through method validation was carried out with these. The method was applied to asparagus samples known as a healthy vegetable being rich in bioactive compounds. Polyphenol contents of asparagus samples were determined by carrying out quantitative and qualitative analyses by LC-DAD-ESI/MS. In this context asparagus sample extracts were obtained using solvents of different polarity. The results were evaluated statistically and showed that rutin is the major phenolic compound in asparagus. This demonstrates the versatility of this rapid and sensitive method for the simultaneous analysis of (poly-)phenolic and flavonoid compounds which was successfully applied to asparagus samples.
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