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Updated: Nov 11, 2025
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
A sensitive immunosensor based on FRET between gold nanoparticles and InP/ZnS quantum dots for arginine kinase
Yanbo Wang1, Junjie Ma1, Huan Li1
1Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
Abstract:
Arginine kinase (AK) is one of the most important allergens in shrimp products. Herein, a novel immunoassay for quantitation of AK was developed using the antibody modified gold nanoparticle (AuNP) and quantum dot (QD). When the first antibody modified AuNP (AuNP-Ab1) was bridged by AK with the secondary antibody modified QD (QD-Ab2), fluorescence resonance energy transfer (FRET) would occur between the AuNP and QD, which led to a decrease in fluorescent signals. The decrease in fluorescence intensity was found to correlate linearly with the log of AK concentration in the range of 1.0 × 10-6-1.0 × 10-3 mg/mL (R2 = 0.9909) and the detection limit was 0.11 ng/mL. The immunoassay was further proved to have encouraging specificity, precision and accuracy. Compared with existing methods, this study provided a promising approach to develop a highly sensitive and selective detection method for AK in shrimp related food samples.
