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Multichannel Paper Chip-Based Gas Pressure Bioassay for Simultaneous Detection of Multiple MicroRNAs.

Lu Shi1, Wei Liu1, Baoxin Li1

  • 1Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.

ACS Applied Materials & Interfaces
|March 24, 2021
PubMed
Summary
This summary is machine-generated.

This study presents a portable, multichannel paper chip bioassay for simultaneous detection of multiple biomarkers using a gas pressure meter. This method offers a simple, low-cost approach for point-of-care diagnostics, improving disease detection efficiency.

Keywords:
microRNAmultichannel paper chipportable gas pressure meterring-oven washing.simultaneous detection

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Area of Science:

  • Biomarker detection
  • Point-of-care diagnostics
  • Biosensor technology

Background:

  • Simultaneous multi-biomarker detection is crucial for accurate disease diagnosis, efficiency, and cost reduction.
  • Portable, simple, and low-cost diagnostics are needed for point-of-care applications in resource-limited settings.
  • Existing methods may lack the required portability, simplicity, or simultaneous detection capabilities.

Purpose of the Study:

  • To develop a multichannel paper chip-based gas pressure bioassay for simultaneous detection of multiple biomarkers.
  • To enable portable, simple, and low-cost point-of-care diagnostics.
  • To improve biomarker detection accuracy and efficiency.

Main Methods:

  • A multichannel paper chip was combined with a portable gas pressure meter.
  • Four DNA tetrahedral probes (DTPs) were immobilized in distinct detection zones for enhanced hybridization and reduced nonspecific adsorption.
  • A sandwich assay format was employed, converting biomolecular recognition into gas pressure signals.
  • Ring-oven washing was utilized for efficient separation of unbound reagents.

Main Results:

  • Simultaneous detection of four lung cancer-related microRNAs (miRNAs) was achieved using the portable gas pressure meter.
  • A linear relationship was observed between gas pressure and the logarithm of miRNA concentration (10 pM to 100 nM).
  • DNA tetrahedral probes demonstrated over a 3-fold improvement in signal-to-noise ratio compared to single-stranded DNA probes.
  • Ring-oven washing provided a cheaper, simpler, and faster separation method than magnetic separation.

Conclusions:

  • The developed multichannel paper chip-based gas pressure bioassay is a promising tool for portable and simultaneous multi-biomarker detection.
  • This assay offers a low-cost, simple, and efficient solution for point-of-care diagnostics.
  • The use of DTPs and ring-oven washing enhances assay performance and practicality.