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FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression
Xiufang Yang1, Huilan Du2, Wenhui Bian3
1Department of Gynecology, Hengshui People's Hospital, Hengshui, Hebei 053000, P.R. China.
Oncology Reports
|March 24, 2021
Summary
Long non-coding RNA FOXD3-AS1 promotes cervical cancer (CC) progression by increasing cell proliferation, migration, and invasion. It upregulates LIMK1 by sponging miR-128-3p, highlighting a new therapeutic target for CC.
Area of Science:
- Molecular Biology
- Oncology
- Gene Regulation
Background:
- Long non-coding RNA (lncRNA) FOXD3-AS1 is implicated as an oncogenic factor in various cancers.
- The specific role and molecular mechanisms of FOXD3-AS1 in cervical cancer (CC) progression remain incompletely elucidated.
- Understanding these mechanisms is crucial for developing targeted therapies for CC.
Purpose of the Study:
- To investigate the biological functions of FOXD3-AS1 in cervical cancer progression.
- To elucidate the potential molecular mechanisms through which FOXD3-AS1 influences CC development.
- To examine the regulatory relationship between FOXD3-AS1, miR-128-3p, and LIMK1 in CC.
Main Methods:
- Quantitative PCR (qPCR) to measure expression levels of FOXD3-AS1, miR-128-3p, and LIMK1.
- Immunohistochemistry and Western blotting to assess LIMK1 protein expression.
- Cell proliferation assays (CCK-8, BrdU), Transwell assays for migration/invasion, and dual-luciferase reporter assays to confirm molecular interactions.
Main Results:
- FOXD3-AS1 expression was significantly elevated in CC tissues and cell lines compared to normal controls.
- High FOXD3-AS1 levels correlated with advanced tumor features, including poor differentiation, larger tumor size, and lymph node metastasis.
- FOXD3-AS1 overexpression promoted CC cell proliferation, migration, and invasion, while knockdown inhibited these processes.
- FOXD3-AS1 directly targeted and downregulated miR-128-3p, leading to the indirect upregulation of LIMK1.
Conclusions:
- FOXD3-AS1 acts as a crucial oncogenic lncRNA in cervical cancer progression.
- The mechanism involves FOXD3-AS1 competitively sponging miR-128-3p, thereby upregulating LIMK1 expression.
- This FOXD3-AS1/miR-128-3p/LIMK1 axis represents a potential therapeutic target for cervical cancer treatment.
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