A novel strategy for enhancing the partial denitrification to treat domestic wastewater by feeding sludge...

Partial-denitrification (PD; NO₃^-→NO₂^-) has recently been proposed to be a feasible choice of NO₂^--N supply for anammox bacteria. In this study, an aerobic/anoxic process for treating domestic wastewater was operated for 176 days to evaluate the feasibility of using sludge fermentation liquid for partial denitrification of the wastewater. Results show that, with the ratio of C/N (COD/ NO₃^--N) increased at anoxic stage, the average NO₂^--N concentration in the effluent and nitrate-to-nitrite transformation ratio (NTR) at anoxic stage showed relative growth. High-throughput sequencing analysis demonstrated that the enhancement of PD can be explained by the increases of Thauera, Paracoccus and Enterobacteriaceae. Moreover, Candidatus_Brocadia (0.13%) was detected as the predominant anammox bacteria. Ex-situ isotopic tracing technique analysis assessed that the ratio of anammox role (ra%) was 7.29%. This study has a great potential for being coupled with the anammox bacteria for advanced nitrogen removal.