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[Experimental transmission of antibiotic resistance in chicks]
Abstract:
Multi-drug resistance of Salmonella heidelberg was transmitted to normal intestinal flora (E. coli organisms were resistant to ampicillin, streptomycin, tetracycline, erythromycin, and oleandomycin. The donor strains of Salmonella heidelberg used in the experiment was a carrier of the following nine markers of resistance: ampicillin, streptomycin, chloramphenicol, kanamycin, neomycin, novobiocin, tetracycline, erythromycin, and oleandomycin. The donor strains of Salmonella heidelberg used in the experiment was a carrier of the following nine markers of resistance: ampicillin, streptomycin, chloramphenicol, kanamycin, neomycin, novobiocin, tetracycline, erythromycin, and oeandomycin, novobiocin, tetracycline, erythromycin, and oleandomycin. The resistance to drugs was transmitted with the oral administration of the donor strain at the preliminary neutralization of the action of hydrochloric acid in the stomah secretion through sodium bicarbonate (1 cm3 of a 10 per cent sol.) an hour prior to feeding the birds with Salmonella heidelberg (1 cm3 of 10(10). In other experiments carboneum tetrachloratum was injected at rates of 0.08 to 0.15 cm3 (acocrting with the body weight of birds) one day prior to infection, followed by the administration of 0.20 to 0.40 cm3 of a 2 per cent solution of omnopon. Escherichia coli organisms acquired new markers of resistance--to chloramphenicol, kanamycin, neomycin and novobiocin. The level of resistance proved equal with that to the donor strain. A total of 1.8 to 5.4 per cent of the intestinal E. coli investigated proved to be carriers of the indicated markers of resitance. Highest level in acquiring markers of multi-drug resistance (13 per cent) showed E. coli organisms isolated from the liver of birds injected additionally with C. tetrachloratum omnopon.
Insights
Multi-drug resistant Salmonella heidelberg transferred resistance markers to E. coli in birds. This transfer occurred via oral administration and was enhanced by carbon tetrachloride and omnopon treatments, increasing E. coli resistance.
Area of Science:
- Microbiology
- Antimicrobial Resistance
- Veterinary Science
Background:
- Salmonella heidelberg can carry multiple drug resistance markers.
- Intestinal flora like E. coli can acquire antibiotic resistance.
- Understanding resistance transfer is crucial for public health.
Purpose of the Study:
- To investigate the transmission of multi-drug resistance from Salmonella heidelberg to E. coli in avian subjects.
- To determine the effectiveness of different administration methods on resistance transfer.
Main Methods:
- Oral administration of Salmonella heidelberg to birds, with prior neutralization of stomach acid.
- Experimental groups received carbon tetrachloride and omnopon treatments.
- Isolation and analysis of E. coli from intestinal flora to detect acquired resistance markers.
Main Results:
- E. coli acquired resistance markers (chloramphenicol, kanamycin, neomycin, novobiocin) from Salmonella heidelberg.
- Resistance levels in E. coli matched the donor strain.
- A higher percentage (13%) of E. coli acquired resistance when birds were pre-treated with carbon tetrachloride and omnopon.
Conclusions:
- Salmonella heidelberg can transfer multi-drug resistance to E. coli in the avian gut.
- Pre-treatment with specific agents can enhance the rate of resistance transfer.
- This highlights a potential pathway for the spread of antimicrobial resistance in animal populations.