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Measuring Organ-Specific Translation Elongation Rate in Mice.
Maxim V Gerashchenko1, Vadim N Gladyshev2
1Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 25, 2021
Summary
This study introduces a novel ribosome profiling method to monitor translation in live mice, enabling the study of protein synthesis regulation in vivo. This technique allows for the analysis of translation elongation and upstream reading frames in intact animals.
Area of Science:
- Molecular Biology
- Genomics
- Animal Models
Background:
- Genome editing facilitates rapid generation of mouse models for protein synthesis studies.
- Studying translation in rodents is challenging due to limited experimental tools and animal complexity.
Purpose of the Study:
- To describe a novel method for monitoring translation in mice and other small animals.
- To enable the study of translation elongation and short upstream reading frames in vivo.
Main Methods:
- Ribosome profiling adapted for small animals.
- Method designed to measure translation elongation.
- Applicable for discovery of short upstream reading frames.
Main Results:
- The method allows for translation monitoring in fully developed animals.
- Preserves unperturbed physiological conditions by avoiding cell extraction.
- Facilitates in vivo studies of protein synthesis regulation.
Conclusions:
- The developed ribosome profiling technique offers a valuable tool for studying translation in intact small animals.
- This method overcomes limitations of current approaches, enabling research under physiological conditions.
- Opens new avenues for investigating gene expression regulation in vivo.
