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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

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Related Experiment Video

Updated: Nov 11, 2025

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

Active Ribosome Profiling with RiboLace: From Bench to Data Analysis.

Massimiliano Clamer¹, Fabio Lauria², Toma Tebaldi³

¹IMMAGINA Biotechnology S.r.l., Povo, Italy. mclamer@immaginabiotech.com.

Methods in Molecular Biology (Clifton, N.J.)

|March 25, 2021

Summary

This summary is machine-generated.

RiboLace is a novel method that isolates active ribosomes for precise translation studies. This technique provides accurate snapshots of ribosome activity genome-wide, correlating well with protein levels.

Keywords:

Codon usage Computational analysis Deep sequencing Polysome RNA Ribosome Ribosome profiling Start codon Translation Translational control

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Last Updated: Nov 11, 2025

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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

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Published on: October 7, 2021

Area of Science:

Molecular Biology
Genomics
Translational Control

Background:

Ribosome profiling is a key technique for studying translation at single-nucleotide resolution.
Classical methods cannot differentiate between active and inactive ribosomes.
Understanding active translation is crucial for deciphering gene expression regulation.

Purpose of the Study:

To introduce RiboLace, a new method for isolating actively translating ribosomes.
To enable accurate genome-wide measurement of translational activity.
To provide a reliable tool for studying translational regulation in biological systems.

Main Methods:

Development of RiboLace, utilizing a puromycin-containing molecule.
Antibody-free and tag-free pull-down approach to isolate active ribosomes.
Application of RiboLace in vitro and in vivo for ribosome footprint analysis.

Main Results:

RiboLace successfully isolates active ribosomes, distinguishing them from inactive ones.
The method achieves high concordance between translational activity and protein levels.
Generated data offers single-nucleotide resolution and genome-wide coverage of active ribosome footprints.

Conclusions:

RiboLace provides a reliable method for estimating the translational state of biological systems.
This technique enhances the analysis of translated genes, translation rates, and ribosome occupancy.
RiboLace offers a powerful new tool for researchers studying gene expression and protein synthesis.