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Updated: Nov 11, 2025

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
Published on: June 23, 2023
Guide to controlling the electron dose to improve low-dose imaging of sensitive samples
1Electron Microscopy Laboratory, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50370, Wroclaw, Poland; Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Weigla 12, 53114 Wroclaw, Poland.
Abstract:
This work describes the methods of beam current measurement and proposes the construction of an easily fabricated Faraday cup for use in any side-entry transmission electron microscope. Moreover, a method of calculating the dose of electrons with the use of a digital camera is proposed and verified with the use of two slightly different microscope setups. The method turned out to be particularly useful for estimating the smallest doses of electrons used in the imaging of particularly sensitive samples.

