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[Arenavirus antigen persistence in continuous cell lines. Attempts at isolating the infectious virus]
Abstract:
An attempt was made to isolate an infectious virus from FL cells in which arenavirus antigen revealed by immunofluorescence tests persisted. Inhibitors of macromolecular synthesis, ultraviolet irradiation, fusion with BHK 21/13S cells, and transfection of BHK-21/13S cells with DNA from FL cells were tested in order to activate the virus. Negative results were obtained in each of these experiments. Prolonged cultivation of FL cells at 31 degres or 41 degrees C resulted in a two-fold increase in proportion of the antigen-containing cells or its complete elimination, respectively. The likelihood of integration of transcribed proviral fragment into the cell genome is discussed.
Insights
Researchers failed to isolate infectious arenavirus from FL cells using various activation methods. Temperature changes affected antigen presence, suggesting potential proviral DNA integration into the cell genome.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Persistent arenavirus antigen was detected in FL cells.
- Previous attempts to isolate infectious virus were unsuccessful.
Purpose of the Study:
- To activate and isolate infectious arenavirus from FL cells.
- To investigate the persistence of arenavirus antigen in FL cells.
Main Methods:
- Tested inhibitors of macromolecular synthesis.
- Used ultraviolet irradiation and cell fusion techniques.
- Performed DNA transfection experiments.
- Cultivated FL cells at different temperatures (31°C and 41°C).
Main Results:
- All attempts to activate infectious virus yielded negative results.
- Cultivation at 31°C increased antigen-containing cells; 41°C eliminated antigen.
- No infectious virus was isolated.
Conclusions:
- Standard methods failed to rescue infectious arenavirus.
- Temperature-dependent modulation of antigen suggests complex viral-cellular interactions.
- Potential integration of proviral fragments into the host cell genome is considered.