Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical outcomes of paediatric home ventilation in South Korea: liberation and mortality.

BMJ open respiratory research·2026
Same author

Microplastics selectively modify ranavirus-driven physiological disruption and gut microbiome restructuring in amphibians.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2026
Same author

A concept mapping study on gender microaggressions experienced by female service members.

Military psychology : the official journal of the Division of Military Psychology, American Psychological Association·2026
Same author

Technology-Dependent Children Living at Home in Korea: A Population-Based Analysis Using NHIS Claims Data.

Journal of Korean medical science·2026
Same author

Recombinant bone morphogenetic protein-2 attenuates colorectal cancer progression by orchestrating Hippo pathway activation, Yes-associated protein inhibition, and epithelial-mesenchymal transition suppression.

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology·2026
Same author

Physician Perceptions Toward Palliative Care Integration in Childhood Cancer Care in Asia Pacific.

JCO global oncology·2026

Related Experiment Video

Updated: Nov 11, 2025

Author Spotlight: Advancing 3D Cytoarchitecture Analysis - Rapid Volumetric Reconstruction of the Human Brain
06:52

Author Spotlight: Advancing 3D Cytoarchitecture Analysis - Rapid Volumetric Reconstruction of the Human Brain

Published on: January 26, 2024

2.5K

Optimizing tissue clearing and imaging methods for human brain tissue.

Min Sun Kim1, Jang Ho Ahn1, Ji Eun Mo1

  • 1Functional Neuroanatomy of Metabolism Regulation Laboratory, Department of Anatomy, Seoul National University College of Medicine, Seoul, South Korea.

The Journal of International Medical Research
|March 27, 2021
PubMed
Summary
This summary is machine-generated.

Fresh human brain samples offer superior clearing and staining efficiency compared to cadaver samples. The 4-day staining with a commercial kit (4DS-C) method provides the deepest penetration for 3D brain visualization.

Keywords:
DAPI stainingGFAP stainingTissue clearingX-CLARITY™confocal microscopyhuman brain

More Related Videos

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
07:20

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

Published on: May 10, 2022

3.2K
Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
08:49

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy

Published on: August 1, 2022

3.9K

Related Experiment Videos

Last Updated: Nov 11, 2025

Author Spotlight: Advancing 3D Cytoarchitecture Analysis - Rapid Volumetric Reconstruction of the Human Brain
06:52

Author Spotlight: Advancing 3D Cytoarchitecture Analysis - Rapid Volumetric Reconstruction of the Human Brain

Published on: January 26, 2024

2.5K
A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
07:20

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

Published on: May 10, 2022

3.2K
Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
08:49

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy

Published on: August 1, 2022

3.9K

Area of Science:

  • Neuroscience
  • Biotechnology
  • Histology

Background:

  • Optimizing human brain tissue preparation is crucial for advanced 3D imaging.
  • Comparing fresh and cadaver samples is essential for protocol development.

Purpose of the Study:

  • To determine optimal sample conditions for human brain clearing and staining.
  • To compare clearing efficiency, antibody staining, and artifacts between fresh and cadaver brain samples.

Main Methods:

  • Human brain samples (fresh and cadaver) were cleared using X-CLARITY™.
  • Clearing efficiency and artifacts were quantified using ImageJ.
  • Antibody staining efficiency was assessed using three methods: 4-day staining (4DS), 11-day staining (11DS), and commercial kit 4-day staining (4DS-C).

Main Results:

  • Fresh samples showed better clearing, fewer artifacts, and superior 4',6-diamidino-2-phenylindole (DAPI) staining efficiency compared to cadaver samples.
  • Glial fibrillary acidic protein (GFAP) staining efficiency was comparable between fresh and cadaver samples.
  • The 4DS-C method yielded the deepest penetration (over 200 µm for immunostaining) within 4 days, enabling 3D visualization of brain structures.

Conclusions:

  • Optimized protocols for human brain sample preparation and staining were developed.
  • These protocols facilitate the visualization of three-dimensional macrostructures in the human brain.