Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromosome Replication02:31

Chromosome Replication

9.7K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
9.7K
The DNA Replication Fork01:02

The DNA Replication Fork

38.5K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
38.5K
The DNA Replication Fork01:02

The DNA Replication Fork

17.0K
17.0K
Replication in Eukaryotes01:29

Replication in Eukaryotes

15.8K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
15.8K
Replication in Eukaryotes02:31

Replication in Eukaryotes

185.2K
Overview
185.2K
Chromosome Structure02:40

Chromosome Structure

24.8K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
24.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting Cancer-Specific Mutations with RNA-Triggered Chromatin Shredding.

Nature·2026
Same author

S-phase checkpoint protects from aberrant replication fork processing and degradation.

Nucleic acids research·2025
Same author

Cell cycle regulation has shaped replication origins in budding yeast.

Nature structural & molecular biology·2025
Same author

Mechanisms for licensing origins of DNA replication in eukaryotic cells.

Nature structural & molecular biology·2025
Same author

Optimization of Enterovirus-like Particle Production and Purification Using Design of Experiments.

Pathogens (Basel, Switzerland)·2025
Same author

Enterovirus-like particles encapsidate RNA and exhibit decreased stability due to lack of maturation.

PLoS pathogens·2025

Related Experiment Video

Updated: Nov 11, 2025

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.4K

DNA replication origins retain mobile licensing proteins.

Humberto Sánchez1, Kaley McCluskey1, Theo van Laar1

  • 1Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, The Netherlands.

Nature Communications
|March 27, 2021
PubMed
Summary
This summary is machine-generated.

Yeast origin recognition complex (ORC) rapidly diffuses along DNA until origin recognition. MCM helicase loading involves ORC-MCM interactions and ATP hydrolysis, resulting in stable MCM loading in multiple forms.

More Related Videos

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.0K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

632

Related Experiment Videos

Last Updated: Nov 11, 2025

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.4K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.0K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

632

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Background:

  • Eukaryotic DNA replication initiates at multiple origins.
  • Origin Recognition Complex (ORC), Cdc6, and Cdt1 recruit the Mcm2-7 (MCM) helicase during G1.
  • Replisome assembly occurs at activated helicase in S phase, but protein dynamics are poorly understood.

Purpose of the Study:

  • Investigate the dynamics of individual proteins during DNA replication initiation.
  • Elucidate the role of protein mobility in the formation of replication complexes.

Main Methods:

  • Single-molecule optical trapping
  • Confocal microscopy
  • Yeast model system

Main Results:

  • Yeast ORC exhibits rapid diffusion along DNA, halted by origin recognition.
  • ORC- and Cdc6-dependent MCM recruitment leads to slow ORC-MCM intermediates and fast-scanning MCMs.
  • ATP hydrolysis results in salt-stable MCM loading in single and double hexamer forms with salt-dependent mobility.

Conclusions:

  • Effective helicase loading depends on the interplay between protein diffusion and origin recognition.
  • MCM is stably loaded onto DNA in multiple forms, suggesting complex regulation of replication initiation.