Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three-dimensional helical integration of high-density linear microelectrode arrays and their cross-tissue applications.

Biosensors & bioelectronics·2026
Same author

Microbiota-fibroblasts interactions in multi-organ fibrosis.

Microbiological research·2026
Same author

Elucidating vadose zone solute transport dynamics <i>via</i> soil-embedded microfluidics: impacts of saturation and heterogeneity.

Lab on a chip·2026
Same author

Losartan shows limited benefit in preclinical models of Geleophysic dysplasia.

Scientific reports·2026
Same author

Ultrasensitive, self-calibrating cortisol immunosensor enabled by dual-modal CuMOF-PEI@AuNPs@HRP immunoreceptor.

Talanta·2026
Same author

Root-knot-nematode-derived mimics of RGF peptides hijack host signalling to orchestrate feeding site formation.

Nature plants·2026

Related Experiment Video

Updated: Nov 11, 2025

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications
11:25

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Published on: April 21, 2016

11.4K

A supersensitive silicon nanowire array biosensor for quantitating tumor marker ctDNA.

Dujuan Li1, Huiyi Chen1, Kai Fan2

  • 1Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, College of Electronics and Information, Hangzhou Dianzi University, Hangzhou, 310018, China.

Biosensors & Bioelectronics
|March 27, 2021
PubMed
Summary
This summary is machine-generated.

A novel silicon nanowire (SiNW) array field-effect transistor (FET) biosensor enables highly sensitive, real-time detection of PIK3CA E542K circulating tumor DNA (ctDNA). This breakthrough offers potential for early cancer diagnosis and monitoring.

Keywords:
Circulating tumor DNA (ctDNA)Field effect transistorPIK3CA E542KSilicon nanowire (SiNW) array biosensor

More Related Videos

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.8K
Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

6.5K

Related Experiment Videos

Last Updated: Nov 11, 2025

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications
11:25

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Published on: April 21, 2016

11.4K
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.8K
Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

6.5K

Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Molecular Diagnostics

Background:

  • Cancer remains a significant global health threat.
  • Circulating tumor DNA (ctDNA) testing via liquid biopsy is crucial for cancer management.
  • Existing ctDNA detection methods require further improvement in sensitivity and real-time analysis.

Purpose of the Study:

  • To develop and characterize a novel field-effect transistor (FET) biosensor for ultrasensitive ctDNA detection.
  • To investigate the real-time, label-free detection capabilities of silicon nanowire (SiNW) arrays for PIK3CA E542K mutations.
  • To assess the biosensor's performance in clinical samples, such as human serum.

Main Methods:

  • Fabrication of a high-response 120-SiNW array using CMOS-compatible microfabrication on a silicon-on-insulator (SOI) substrate.
  • Modification of the SiNW array surface with DNA probes via silanization for specific ctDNA capture.
  • Real-time, label-free electrical detection of ctDNA using the FET biosensor.

Main Results:

  • The SiNW-array FET biosensor achieved an ultralow detection limit of 10 aM for ctDNA.
  • A wide linear detection range from 0.1 fM to 100 pM was demonstrated.
  • The biosensor exhibited high selectivity, distinguishing target ctDNA from mismatched DNA sequences.
  • Successful detection of ctDNA in human serum samples was achieved.

Conclusions:

  • The developed SiNW-array FET biosensor provides a highly sensitive and selective platform for ctDNA detection.
  • This technology shows significant potential for non-invasive cancer diagnosis, targeted therapy selection, and prognosis.
  • The label-free, real-time detection capability makes it suitable for future clinical applications.