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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
Ultrasensitive, self-calibrating cortisol immunosensor enabled by dual-modal CuMOF-PEI@AuNPs@HRP immunoreceptor
Xinyi Wang1, Minghao Wang1, Wenbin Zhang2
1Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, School of Electronics and Information, Hangzhou Dianzi University, Hangzhou, 310018, China; Key Laboratory of Micro-nano Sensing and IoT of Wenzhou, Wenzhou Institute of Hangzhou Dianzi University, Wenzhou, 325038, China.
This study introduces a novel dual-modal immunosensor for accurate cortisol monitoring. The fluorescence-colorimetric sensor enhances reliability and sensitivity for stress assessment and health management.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Materials Science
Background:
- Accurate cortisol monitoring is crucial for stress assessment and health management.
- Conventional single-mode immunosensors face challenges like signal fluctuations and matrix effects, limiting reliability.
- Developing robust and sensitive cortisol detection methods is essential.
Purpose of the Study:
- To develop a reliable dual-modal immunosensor for accurate cortisol determination.
- To overcome the limitations of single-mode immunosensors in cortisol quantification.
- To enhance sensitivity and calibration reliability for stress biomarker monitoring.
Main Methods:
- Fabrication of a fluorescence-colorimetric (FL-CL) dual-modal immunosensor using a CuMOF-PEI@AuNPs@HRP (CPA) immunoreceptor.
- Integration of correlated fluorescence and colorimetric responses from a single recognition event.
- Utilizing a normalized dual-signal output for improved calibration reliability.
Main Results:
- The proposed dual-modal immunosensor achieved a detection limit of 0.22 ng/mL and a linear range of 3.1-200 ng/mL for cortisol.
- The sensor demonstrated enhanced sensitivity, improved correlation, good stability, and batch reproducibility compared to single-mode assays and commercial kits.
- The FL-CL dual-modal approach effectively mitigated signal fluctuations and matrix effects.
Conclusions:
- The developed FL-CL dual-modal immunosensor provides a reliable and sensitive strategy for cortisol monitoring in saliva.
- The integrated sensing platform offers potential for extension to the detection of other biomarkers.
- This work advances the development of advanced diagnostic tools for health management and stress assessment.
