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Updated: Nov 11, 2025

Development of Leishmania Species Strains with Constitutive Expression of eGFP
Published on: April 21, 2023
Raising the Bar(-seq) in Leishmania Genetic Screens
Tiago R Ferreira1, Rafael M Couñago2, Nilmar S Moretti3
1Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD, USA.
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Our understanding of regulatory factors in Leishmania differentiation has long been restricted by the available genetic tools, but the availability of CRISPR/Cas9 has changed the landscape forever. Recently, Baker and Catta-Preta et al. applied Cas9 editing and kinome-wide bar-seq to dissect the function of 204 kinases in the Leishmania mexicana life cycle.
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