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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Nov 11, 2025

Author Spotlight: Development of a Method for Identifying Small Molecular Antagonists of β2 Integrin Activation

Author Spotlight: Development of a Method for Identifying Small Molecular Antagonists of β2 Integrin Activation

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Protocol for high-throughput compound screening using flow cytometry in THP-1 cells.

Stephan H Spangenberg¹, Reza Beheshti Zavareh¹, Luke L Lairson¹

¹Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

|March 29, 2021

Summary

This study presents a high-throughput flow cytometry protocol for screening small molecules that regulate PD-L1 expression in THP-1 cells. This method efficiently analyzes large compound libraries for potential therapeutic targets.

Keywords:

Cell-based assays Flow cytometry/mass cytometry High-throughput screening Immunology

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Last Updated: Nov 11, 2025

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Published on: March 10, 2018

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Flow cytometry is crucial for single-cell protein expression analysis.
Analyzing large sample numbers with flow cytometry presents challenges.
THP-1 cells, a human monocytic leukemia cell line, are used.

Purpose of the Study:

To develop a high-throughput protocol for small molecule screening using flow cytometry.
To identify compounds regulating PD-L1 surface expression in IFN-γ-stimulated THP-1 cells.

Main Methods:

Utilized flow cytometry for analyzing protein expression at the single-cell level.
Developed a methodology for high-throughput screening of small molecules.
Employed THP-1 cells and IFN-γ stimulation.

Main Results:

Successfully screened a collection of approximately 200,000 compounds.
Identified compounds that modulate PD-L1 surface expression.

Conclusions:

The described protocol enables efficient high-throughput screening of small molecules.
This method is valuable for identifying regulators of PD-L1 expression in immune cells.