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Stability of IgE in Postmortem Plasma and Hemolyzed Samples
T Q Wang1, T F Wang2, Y Q Jia1
1School of Forensic Medicine, China Medical University, Shenyang 110122, China.
Fa Yi Xue Za Zhi
|March 29, 2021
Summary
Immunoglobulin E (IgE) shows good stability in postmortem plasma and hemolyzed samples, especially in plasma, which is recommended for forensic analysis. This finding is crucial for accurate postmortem diagnostics.
Area of Science:
- Forensic Science
- Immunology
- Biochemistry
Background:
- Accurate postmortem diagnostics rely on the stability of biomarkers like Immunoglobulin E (IgE).
- Understanding IgE stability in postmortem samples is critical for reliable forensic investigations.
Purpose of the Study:
- To assess the stability of IgE in postmortem plasma and hemolyzed samples.
- To evaluate IgE stability under various storage conditions (temperature, duration) and freeze-thaw cycles.
Main Methods:
- Collected 39 postmortem blood samples (plasma and hemolyzed).
- Stored samples at -20°C, 4°C, and 25°C for 28 days, and at -80°C for 1 year.
- Subjected samples to 5 freeze-thaw cycles and measured IgE concentration using electroluminescence.
Main Results:
- IgE showed good stability in plasma at -80°C for 1 year and after 5 freeze-thaw cycles.
- Hemolyzed samples exhibited faster IgE degradation than plasma, particularly at 25°C and after 1 year at -80°C.
- Degradation rates were comparable across -20°C, 4°C, and 25°C for plasma over 28 days.
Conclusions:
- IgE is stable in postmortem plasma and hemolyzed samples, with superior stability observed in plasma.
- Prompt plasma separation from postmortem blood is recommended in forensic practice to ensure IgE integrity.
