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Updated: Nov 11, 2025
Purification of a High Molecular Mass Protein in Streptococcus mutans
Published on: September 14, 2019
Fluoride Binding to Streptococcus mutans Pellets Rich in Extracellular Polysaccharides
Leonardo Libardi Pagotto1, Manuela da Silva Spinola2, Diego Figueiredo Nóbrega3
1Department of Biosciences, Piracicaba Dental School, University of Campinas, São Paulo, Brazil.
Abstract:
Extracellular polysaccharides (EPS), mainly the insoluble ones, increase the cariogenicity of dental biofilm, but whether they interfere with the binding and retention of fluoride is unknown. EPS-rich (EPS+) and EPS-poor (EPS-) pellets of Streptococcus mutans were formed and treated with increasing fluoride concentrations (0, 0.1, 1, or 10 mM). A concentration-dependent fluoride binding was observed in both EPS- and EPS+ pellets, but the presence of EPS did not affect the retention of fluoride in the pellets. In conclusion, the data suggest that a matrix of dental biofilm rich in EPS does not affect fluoride retention in the biofilm.
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