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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
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[CRISPR/Cas-based genome editing in Aspergillus niger].

Xiaomei Zheng1,2,3,4, Ping Zheng1,2,3,4, Jibin Sun1,2,3,4

  • 1Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|March 30, 2021
PubMed
Summary
This summary is machine-generated.

Aspergillus niger is a key fungus for industrial production. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated (Cas) system revolutionizes its genetic modification for enhanced cell factory applications.

Keywords:
Aspergillus nigerCRISPR/Cas systemcell factorygenome editing

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Area of Science:

  • Biotechnology
  • Industrial Microbiology
  • Molecular Biology

Background:

  • Aspergillus niger is a widely utilized fungus in industrial biotechnology, recognized for its GRAS status and robust fermentation capabilities.
  • It serves as a crucial cell factory for producing organic acids and industrial enzymes.
  • Advances in synthetic and systems biology are enhancing our understanding and optimization of A. niger.

Purpose of the Study:

  • To review the advancements in Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated (Cas) genome editing technology.
  • To highlight the applications of CRISPR/Cas in modifying genes and regulating gene expression in Aspergillus niger.
  • To discuss future prospects of CRISPR/Cas genome editing for A. niger.

Main Methods:

  • Review of current literature on CRISPR/Cas applications in Aspergillus niger.
  • Analysis of gene modification strategies using CRISPR/Cas.
  • Examination of gene expression regulation techniques facilitated by CRISPR/Cas.

Main Results:

  • The CRISPR/Cas system represents a significant breakthrough for targeted genome modification in A. niger.
  • This technology enables precise gene editing and effective regulation of gene expression.
  • The review details various applications and successful implementations of CRISPR/Cas in A. niger.

Conclusions:

  • CRISPR/Cas technology is revolutionizing the genetic engineering of Aspergillus niger.
  • It offers powerful tools for optimizing A. niger as an industrial cell factory.
  • Future research directions focus on expanding the CRISPR/Cas toolbox for A. niger.