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Solid-phase radioimmunoassay of human complement fragment C5a
S Chang1, A Leo-Mensah, J Campbell
1Ciba-Corning Diagnostics, E. Walpole, MA 02032.
Clinical Chemistry
|June 1, 1988
Abstract:
In this competitive RIA for determining concentrations of human C5a in biological fluids and in buffers, labeled C5a and sample are allowed to compete for binding to a limited amount of goat antibody to human C5a in solution. Free and bound tracer are then separated by a second antibody (rabbit anti-goat IgG) immobilized on paramagnetic particles. Total incubation time for this assay is 70 min. Sensitivity, precision, and analytical recovery of this assay compare well with those of a reference method.