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Related Experiment Videos

Development of standard materials for use with the hematofluorometer.

P J Parsons1, J R Meola, D G Mitchell

  • 1Laboratory of Inorganic and Nuclear Chemistry, Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.

Clinical Chemistry
|June 1, 1988
PubMed
Summary

Stable blood standards for hematofluorometers were developed using two erythrocyte preparation methods. Plasma-free erythrocytes in citrate-glycerol solution offered superior 18-week stability for proficiency testing.

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Area of Science:

  • Hematology
  • Clinical Chemistry
  • Biostability Studies

Background:

  • Hematofluorometers require stable, blood-based standard materials for accurate measurements.
  • Current erythrocyte preparation methods may lack sufficient long-term stability for reliable calibration and quality control.

Purpose of the Study:

  • To evaluate two distinct erythrocyte preparation techniques for developing stable blood-based standards.
  • To determine the optimal method for achieving long-term storage stability of erythrocytes for hematofluorometer applications.

Main Methods:

  • Erythrocytes were washed using either citrate-phosphate-dextrose (CPD) solution or isotonic saline.
  • Washed erythrocytes were reconstituted with either platelet-free plasma or a citrate-glycerol solution.
  • Prepared erythrocyte samples were stored at 4 degrees C and assessed for stability over time.

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Main Results:

  • Erythrocytes prepared with CPD solution and platelet-free plasma demonstrated stability for 12 weeks.
  • Plasma-free erythrocytes washed with isotonic saline and resuspended in citrate-glycerol solution exhibited enhanced stability, lasting for 18 weeks at 4 degrees C.
  • The 18-week stable erythrocyte preparation was successfully implemented as proficiency test specimens.

Conclusions:

  • Plasma-free erythrocytes suspended in citrate-glycerol solution provide a highly stable blood-based material for hematofluorometer calibration.
  • This optimized preparation method significantly improves the reliability of hematofluorometer proficiency testing programs.
  • The developed standard material enhances the accuracy and consistency of erythrocyte measurements in clinical laboratories.