Related Experiment Video
Updated: Nov 11, 2025
09:25
Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
Published on: November 1, 2024
2.3K
NORD: NO Relaxation Delay NMR Spectroscopy
Tamás Milán Nagy1, Katalin E Kövér1,2, Ole W Sørensen3
1Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem tér 1, 4032, Debrecen, Hungary.
Angewandte Chemie (International Ed. in English)
|March 30, 2021
Summary
Novel NO relaxation delay (NORD) NMR spectroscopy accelerates data acquisition by enabling magnetization relaxation during concatenated experiments. This technique efficiently generates detailed correlation maps for complex molecules, saving significant instrument time.
Area of Science:
- Chemistry
- Spectroscopy
- Nuclear Magnetic Resonance (NMR)
Background:
- NMR spectroscopy is crucial for molecular structure elucidation.
- Traditional NMR experiments can be time-consuming, limiting throughput.
- Optimizing instrument time is essential for efficient research.
Purpose of the Study:
- Introduce the novel NO relaxation delay (NORD) NMR spectroscopy concept.
- Demonstrate NORD's ability to reduce experimental time.
- Showcase NORD's applicability to complex samples.
Main Methods:
- Designing concatenated NMR experiments.
- Utilizing magnetization relaxation during subsequent experiments.
- Applying NORD to trisaccharide and hydrocortisone samples.
Main Results:
- Achieved complete, well-resolved 1H-1H and 1H-13C correlation maps.
- Acquired data for an 80 mM trisaccharide solution in under two minutes.
- Successfully applied NORD to hydrocortisone despite spectral overlap.
Conclusions:
- NORD NMR spectroscopy significantly reduces experiment duration.
- The technique is effective for generating detailed correlation maps.
- NORD offers a valuable approach for rapid analysis of complex molecules.
More Related Videos
![Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F59399.jpg&w=3840&q=50)
Related Concept Videos
01:28Atomic Nuclei: Types of Nuclear Relaxation
595
Nuclear relaxation restores the equilibrium population imbalance and can occur via spin–lattice or spin–spin mechanisms, which are first-order exponential decay processes.
In spin–lattice or longitudinal relaxation, the excited spins exchange energy with the surrounding lattice as they return to the lower energy level. Among several mechanisms that contribute to spin–lattice relaxation, magnetic dipolar interactions are significant. Here, the excited nucleus transfers...
In spin–lattice or longitudinal relaxation, the excited spins exchange energy with the surrounding lattice as they return to the lower energy level. Among several mechanisms that contribute to spin–lattice relaxation, magnetic dipolar interactions are significant. Here, the excited nucleus transfers...
595
01:14NMR Spectrometers: Resolution and Error Correction
903
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
903
01:15¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
1.3K
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
1.3K
01:02¹H NMR: Interpreting Distorted and Overlapping Signals
1.2K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.2K
00:52¹H NMR of Conformationally Flexible Molecules: Temporal Resolution
1.1K
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
1.1K
01:15NMR Spectroscopy: Chemical Shift Overview
2.6K
The position of the absorption signal of a sample is reported relative to the position of the signal of tetramethylsilane (TMS), which is added as an internal reference while recording spectra. The difference between the absorption frequencies of the sample and TMS (in Hz) is divided by the spectrometer operating frequency (in MHz) to obtain a dimensionless quantity called the chemical shift. It is reported on the δ (delta) scale and expressed in parts per million.
For instance, the proton...
For instance, the proton...
2.6K