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Systems for in vivo hypermutation: a quest for scale and depth in directed evolution

Gordon Rix1, Chang C Liu2

  • 1Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, 92697, USA; Center for Synthetic Biology, The Henry Samueli School of Engineering, University of California, Irvine, CA, 92697, USA.

Current Opinion in Chemical Biology
|March 30, 2021
PubMed
Summary

Engineered genetic systems enable continuous evolution by increasing gene mutation rates in vivo. This approach overcomes limitations of traditional methods, expanding the scale and depth of evolutionary searches for genes of interest.

Keywords:
Continuous evolutionDirected evolutionHypermutationProtein evolution

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