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ICG Lymphography in a 4-week Postmortem Cadaver: Implications for a Supermicrosurgery Training Model.
Elizabeth G Zolper1, Jenna C Bekeny1, Kenneth L Fan1
1Department of Plastic and Reconstructive Surgery, MedStar Georgetown University Hospital, Washington, DC.
Plastic and Reconstructive Surgery. Global Open
|March 31, 2021
Summary
Supermicrosurgery training models are limited. Researchers successfully used a fresh, frozen cadaver for indocyanine green (ICG) lymphography, visualizing the lymphatic system for improved surgical simulation.
Area of Science:
- Surgical innovation
- Medical training models
- Supermicrosurgery
Background:
- Surgical models are crucial for training and innovation.
- Current supermicrosurgery (SM) models are limited and imperfect.
- Cadaveric specimens offer potential for advanced surgical training.
Purpose of the Study:
- To report a novel method for lymphatic mapping in cadaveric specimens.
- To evaluate the utility of a fresh, previously frozen cadaver for supermicrosurgery simulation.
- To establish a reproducible technique for indocyanine green (ICG) lymphography in upper extremity cadavers.
Main Methods:
- Utilized a fresh, previously frozen 4-week postmortem cadaveric upper extremity specimen.
- Performed distal to proximal indocyanine green (ICG) lymphography.
- Confirmed lymphatic visualization using handheld SPY fluorescence imaging.
Main Results:
- Successfully demonstrated ICG lymphography in the cadaveric specimen.
- Visualized a clearly defined, linear lymphatic system via fluorescence imaging.
- Validated the feasibility of using this cadaveric model for SM training.
Conclusions:
- A straightforward and reproducible method for lymphatic mapping in cadaveric specimens was established.
- This technique expands the available surgical models for supermicrosurgery training and research.
- Fresh, frozen cadavers are viable for advanced lymphatic mapping simulation in SM.

