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Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System
Published on: July 31, 2019
Genomics and functional genomics in Leishmania and Trypanosoma cruzi: statuses, challenges and perspectives
Daniella C Bartholomeu1, Santuza Maria Ribeiro Teixeira2, Angela Kaysel Cruz3
1Universidade Federal de Minas Gerais, Departamento de Parasitologia, Belo Horizonte, MG, Brasil.
Abstract:
The availability of Trypanosomatid genomic data in public databases has opened myriad experimental possibilities that have contributed to a more comprehensive understanding of the biology of these parasites and their interactions with hosts. In this review, after brief remarks on the history of the Trypanosoma cruzi and Leishmania genome initiatives, we present an overview of the relevant contributions of genomics, transcriptomics and functional genomics, discussing the primary obstacles, challenges, relevant achievements and future perspectives of these technologies.
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