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Multi-Locus Sequencing for Strain Typing of Toxoplasma gondii
Published on: May 26, 2026
Development of a novel SNV-based genotyping method for Prototheca bovis
Masato Akiba1, Minori Onaga1, Tsuyoshi Sekizuka2
1School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Journal of Microbiological Methods
|September 6, 2026
Abstract:
We developed a novel single nucleotide variant (SNV)-based genotyping method for Prototheca bovis based on mitochondrial and plastid genome polymorphisms. Sequencing of five PCR amplicons targeting six informative SNVs discriminated field isolates with a Simpson's D value of 0.927, providing a simple and robust epidemiological typing method.

