Measurement of phosphatases in biological fluids

Electrophoretic and immunological studies are beginning to allow a detailed picture to emerge for the isoenzymes of both acid and alkaline phosphatase. Such findings are occasionally in conflict with the time-honored biochemical methods considered to revealthe organ of origin of a given isoenzyme. In this review, recent reports describing electrophoretic and biochemical studies are correlated, and an attempt is made to clarify the useful clinical significance of enzyme levels determined by the various chemical and electrophoretic methods.