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Related Experiment Video

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Caffeine improves bladder function in diabetic rats via a neuroprotective effect.

Jun Xue1, Yadong Liu1, Sichong Zhang1

  • 1Department of Urology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210011, P.R. China.

Experimental and Therapeutic Medicine
|April 1, 2021
PubMed
Summary

Caffeine improves bladder function in diabetic rats by protecting dorsal root ganglion nerves. This study investigated the mechanisms, finding caffeine beneficial for diabetic cystopathy symptoms and nerve cell survival.

Keywords:
bladder functioncaffeinediabetes mellitusdorsal root ganglion

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Area of Science:

  • Urology
  • Neuroscience
  • Pharmacology

Background:

  • Diabetic cystopathy (DCP) is a common complication of diabetes mellitus (DM).
  • Previous research suggests caffeine may alleviate bladder dysfunction in diabetic rats.
  • The precise mechanisms of caffeine's effects on DCP require further investigation.

Purpose of the Study:

  • To elucidate the mechanisms by which caffeine improves bladder function in a rat model of diabetes mellitus.
  • To assess the impact of caffeine on nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and calcitonin gene-related peptide (CGRP) levels.
  • To evaluate caffeine's effect on apoptosis in the dorsal root ganglion (DRG) and related protein expression.

Main Methods:

  • Urodynamic analyses were performed to measure bladder function in control, caffeine, DM, and DM + caffeine groups.
  • ELISA was used to quantify NGF, BDNF, and CGRP levels in bladder tissue.
  • Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay and Western blotting were employed to assess DRG apoptosis and protein expression (Bcl-2, Bax, caspases).

Main Results:

  • Caffeine treatment improved urination time, micturition interval, and maximum voiding pressure while decreasing bladder wet weight in diabetic rats.
  • Elevated levels of NGF, BDNF, and CGRP were observed in the bladder tissue of caffeine-treated diabetic rats.
  • Caffeine administration reduced DRG cellular apoptosis and modulated the expression of Bcl-2, Bax, cleaved caspase-3, and cleaved caspase-9 proteins.

Conclusions:

  • Caffeine demonstrates a protective effect on the dorsal root ganglion (DRG) in diabetic rats.
  • These neuroprotective mechanisms contribute to the observed improvements in bladder function in diabetic cystopathy.
  • Caffeine represents a potential therapeutic agent for managing diabetic cystopathy.