Fraction collector for capillary zone electrophoresis

Area of Science:

• Analytical Chemistry
• Biochemistry
• Biophysical Chemistry

Background:

• Capillary zone electrophoresis (CZE) is a powerful separation technique.
• Studying the impact of CZE on biomolecules requires specialized collection methods.
• Previous methods may not adequately preserve biological activity post-separation.

Purpose of the Study:

• To describe a novel fraction collector for capillary zone electrophoresis.
• To characterize the collector's ability to discretely isolate separated sample components.
• To investigate the effect of CZE separation on the biological activity of alpha-chymotrypsin.

Main Methods:

• Development and implementation of a novel fraction collector for CZE.
• Characterization of the fraction collector's performance in discrete sample collection.
• Assay of alpha-chymotrypsin biological activity after CZE separation using the developed collector.

Main Results:

• The described instrument effectively collects discrete fractions from CZE separations.
• The fraction collector allows for the study of separated components.
• Initial studies indicate the potential to assess CZE's impact on enzyme activity.

Conclusions:

• A functional fraction collector for CZE has been developed.
• This tool facilitates the analysis of separated analytes.
• The system enables research into the effects of CZE on biomolecular function, specifically alpha-chymotrypsin.