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Updated: Nov 10, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
Published on: April 14, 2015
Stem-Loop qRT-PCR-Based Quantification of miRNAs
Yoann Abel1, Mathieu Rederstorff2
1IGMM, CNRS, Université de Montpellier, Montpellier, France. yoann.abel@igmm.cnrs.fr.
Abstract:
Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR. This fast and simple method provides quantitative data with high sensitivity and specificity to study miRNAs and their functions.
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