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Related Experiment Video

Updated: Nov 10, 2025

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Next generation sequencing in cytology.

Pasquale Pisapia¹, Francesco Pepe¹, Roberta Sgariglia¹

¹Department of Public Health, University of Naples Federico II, Naples, Italy.

Cytopathology : Official Journal of the British Society for Clinical Cytology

|April 1, 2021

Summary

Next-generation sequencing (NGS) of cytological samples offers valuable insights for targeted treatments and cancer risk assessment. Pre-analytical factors are critical for ensuring the reliability of these molecular cytopathology analyses.

Keywords:

FNA biomarkers cytopathology molecular cytopathology next generation sequencing

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High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

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Last Updated: Nov 10, 2025

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Published on: April 11, 2016

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Published on: March 21, 2018

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

Area of Science:

Molecular Cytopathology
Genomics
Biotechnology

Background:

Next-generation sequencing (NGS) is transforming molecular cytopathology.
Cytological samples are a rich source of high-quality DNA for NGS.
NGS aids in predicting treatment response and assessing malignancy risk.

Purpose of the Study:

To review challenges associated with NGS in cytology.
To identify pre-analytical factors impacting NGS reliability in cytological samples.
To discuss future directions for NGS in routine diagnostic applications.

Main Methods:

Review of current literature on NGS in cytopathology.
Focus on pre-analytical variables affecting DNA quality and NGS outcomes.
Analysis of factors influencing diagnostic accuracy and success rates.

Main Results:

Cytological samples provide high-quality DNA suitable for NGS.
Pre-analytical factors significantly influence the reliability of NGS results.
Addressing these factors is crucial for successful clinical implementation.

Conclusions:

NGS of cytological samples holds significant promise for personalized medicine.
Careful attention to pre-analytical steps is essential to overcome current challenges.
Further research and standardization will enhance routine diagnostic applications.