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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

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Design and analytical validation of Nexthyro 2.0, a custom next-generation sequencing panel integrating DNA mutation

Mariantonia Nacchio1, Anna Maria Carillo1, Domenica Di Giovanni1

  • 1Department of Public Health, Pathology Division, University of Naples Federico II, Naples, Italy.

Virchows Archiv : an International Journal of Pathology
|August 31, 2026
PubMed

Keywords:
Analytical validationFine-needle aspirationMolecular diagnosticsNext-generation sequencingThyroid cytology

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Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.