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Related Experiment Video

Updated: Nov 10, 2025

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

Molecular-scale axial localization by repetitive optical selective exposure.

Lusheng Gu^1,2,3,4,5, Yuanyuan Li^1,2,5, Shuwen Zhang^1,2,4,6

¹National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

|April 2, 2021

Summary

We developed a new super-resolution imaging technique called repetitive optical selective exposure (ROSE-Z). This method achieves unprecedented axial localization precision with minimal photons, improving cellular nanostructure investigation.

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Last Updated: Nov 10, 2025

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

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Published on: October 13, 2011

Patterning via Optical Saturable Transitions - Fabrication and Characterization

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Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

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Published on: June 30, 2018

Area of Science:

Optics and Photonics
Biophysics
Microscopy

Background:

Super-resolution microscopy is crucial for visualizing cellular nanostructures.
Existing methods face limitations in axial localization precision and photon efficiency.
Astigmatism-based methods are common but can be photon-intensive.

Purpose of the Study:

To introduce a novel axial localization method for super-resolution imaging.
To enhance precision and photon efficiency in 3D nanoscale imaging.
To facilitate detailed investigation of cellular nanostructures.

Main Methods:

Development of the axial localization with repetitive optical selective exposure (ROSE-Z) method.
Utilizing an asymmetric optical scheme to generate interference fringes.
Demonstration of nanoscale three-dimensional and two-color imaging.

Main Results:

Achieved <2 nm axial localization precision.
Required only ~3,000 photons for high precision.
Demonstrated a sixfold improvement over previous astigmatism methods.
Successfully performed nanoscale 3D and two-color imaging.

Conclusions:

The ROSE-Z method offers superior performance in super-resolution imaging.
This technique significantly improves axial localization precision and photon efficiency.
ROSE-Z facilitates advanced studies of cellular nanostructures.