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Harnessing developmental plasticity to pattern kidney organoids.

Rohan Bhattacharya1, Makenzie G Bonner2, Samira Musah3

  • 1Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA; Center for Biomolecular and Tissue Engineering, Duke University, Durham, NC, USA.

Cell Stem Cell
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Summary
This summary is machine-generated.

Researchers identified distinct cell populations in human stem cell-derived kidney tissues. These cells could be guided to form ureteric epithelium (UE), aiding kidney development studies.

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Area of Science:

  • Developmental Biology
  • Stem Cell Research
  • Urology

Background:

  • Understanding kidney development is crucial for regenerative medicine.
  • Deriving specific kidney cell types in vitro presents a significant challenge.
  • The ureteric epithelium (UE) plays a key role in kidney patterning.

Purpose of the Study:

  • To identify distinct cell populations within human induced pluripotent stem cell (iPSC)-derived distal nephron (DN) epithelia.
  • To determine if these cell populations can be induced to a UE phenotype.
  • To advance methods for in vitro derivation of UE for kidney organoid studies.

Main Methods:

  • Utilized human induced pluripotent stem cells (iPSCs).
  • Generated kidney organoids and distal nephron epithelia.
  • Performed transcriptional profiling to identify distinct cell populations.
  • Inducible differentiation protocols were applied.

Main Results:

  • Identified transcriptionally distinct cell populations within iPSC-derived DN epithelia.
  • Demonstrated the inducibility of these cells towards a UE phenotype.
  • Successfully generated UE-like cells within the context of kidney organoids.

Conclusions:

  • Human iPSC-derived DN epithelia contain cells amenable to UE differentiation.
  • This finding provides a novel strategy for generating UE in vitro.
  • Advances the potential for studying kidney development and disease using organoids.