Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circumferential strain recovery after human cardiomyocyte transplantation in minipigs using a novel frequency-based method for myocardial tagging quantification.

Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance·2026
Same author

Human Multi-Organ-on-a-Chip Platforms for Next-Generation Drug Delivery Strategies.

Theranostics·2026
Same author

Immune-competent new approach methodologies for a hybrid future.

Nature immunology·2026
Same author

Purinergic signaling promotes gliomagenesis through nuclear calcium transients.

bioRxiv : the preprint server for biology·2026
Same author

Label-free quantitative 3D mapping of collagen architecture by holotomography.

bioRxiv : the preprint server for biology·2026
Same author

Toward bioengineered muscle-fat microphysiological systems for sports medicine and obesity therapeutics.

Advanced drug delivery reviews·2026

Related Experiment Video

Updated: Nov 10, 2025

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
08:41

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

9.3K

Tunable electroconductive decellularized extracellular matrix hydrogels for engineering human cardiac

Jonathan H Tsui1, Andrea Leonard2, Nathan D Camp3

  • 1Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21205, USA.

Biomaterials
|April 2, 2021
PubMed
Summary

Hybrid hydrogels combining decellularized extracellular matrix and reduced graphene oxide mature human induced pluripotent stem cell-derived cardiomyocytes. This biomaterial enhances engineered heart tissue function and drug response prediction.

Keywords:
BioprintingCardiac tissue engineeringDecellularized extracellular matrixGraphene oxideHybrid materials

More Related Videos

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
06:17

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells

Published on: March 28, 2025

777
Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair
05:05

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

Published on: June 9, 2020

5.7K

Related Experiment Videos

Last Updated: Nov 10, 2025

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
08:41

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

9.3K
3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
06:17

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells

Published on: March 28, 2025

777
Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair
05:05

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

Published on: June 9, 2020

5.7K

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Cardiovascular Research

Background:

  • Human induced pluripotent stem cells (hiPSCs) are crucial for engineered heart tissues (EHTs) but exhibit phenotypic immaturity.
  • This immaturity limits the reliability of in vitro drug screening and disease modeling.
  • Developing instructive microenvironments is key to overcoming these limitations.

Purpose of the Study:

  • To engineer advanced hybrid hydrogels using decellularized porcine myocardial extracellular matrix (dECM) and reduced graphene oxide (rGO).
  • To create a biomaterial that promotes the maturation of hiPSC-derived cardiomyocytes within EHTs.
  • To enhance the functional and electrophysiological properties of EHTs for improved drug response assessment.

Main Methods:

  • Fabrication of dECM-rGO hybrid hydrogels with tunable mechanical and electrical properties.
  • Encapsulation of hiPSC-derived cardiomyocytes within dECM-rGO scaffolds to form EHTs.
  • Assessment of EHT contractile function, gene expression, and electrophysiological properties.
  • Utilization of dECM-rGO EHTs for high-throughput drug screening, specifically evaluating cisapride's proarrhythmic potential.

Main Results:

  • The dECM-rGO hydrogels preserved a tissue-specific protein profile and allowed property modulation.
  • EHTs cultured in dECM-rGO scaffolds showed significantly increased twitch forces and enhanced expression of contractile function genes.
  • Significant improvements in electrophysiological functions, including calcium handling, action potential duration, and conduction velocity, were observed.
  • dECM-rGO EHTs accurately predicted cisapride's proarrhythmic effects, showing action potential prolongation and beat interval irregularities at clinical doses.

Conclusions:

  • Hybrid dECM-rGO hydrogels provide an instructive microenvironment that promotes hiPSC-cardiomyocyte maturation and EHT development.
  • These advanced EHTs exhibit enhanced physiological function and are capable of producing reliable drug responses.
  • The dECM-rGO system offers a promising platform for high-throughput drug screening and disease modeling in cardiovascular research.