Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

23.6K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
23.6K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.4K
4.4K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.6K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

9.3K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.3K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.1K
3.1K
RNA Splicing01:32

RNA Splicing

58.5K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
58.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A comprehensive tRNA pseudouridine map uncovers targets dependent on human stand-alone pseudouridine synthases.

Nature cell biology·2025
Same author

The 2024 Nobel Prize in Physiology or Medicine: microRNA Takes Center Stage.

Non-coding RNA·2024
Same author

Going circular: history, present, and future of circRNAs in cancer.

Oncogene·2023
Same author

Chromatin interaction maps identify Wnt responsive cis-regulatory elements coordinating Paupar-Pax6 expression in neuronal cells.

PLoS genetics·2022
Same author

RNA thermometers and other regulatory elements: Diversity and importance in bacterial pathogenesis.

Wiley interdisciplinary reviews. RNA·2022
Same author

Post-Transcriptional Regulation through Long Non-Coding RNAs (lncRNAs).

Non-coding RNA·2021

Related Experiment Video

Updated: Nov 10, 2025

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.1K

Epigenetic Regulation of Alternative Splicing: How LncRNAs Tailor the Message.

Giuseppina Pisignano1, Michael Ladomery2

  • 1Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK.

Non-Coding RNA
|April 3, 2021
PubMed
Summary

Long non-coding RNAs (lncRNAs) significantly regulate alternative splicing, a key process for proteomic diversity. This review highlights recent findings on how lncRNAs shape splice isoform expression, impacting cellular functions and disease.

Keywords:
alternative splicinglong non-coding RNAspost-transcriptional regulationsplicing factors

More Related Videos

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.2K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.2K

Related Experiment Videos

Last Updated: Nov 10, 2025

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.1K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.2K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Alternative splicing generates proteomic diversity in eukaryotes, with most human genes exhibiting cell-type-specific splicing.
  • Dysregulation of alternative splicing is linked to altered RNA/protein functions and various diseases.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in regulating cellular processes, including alternative splicing.

Purpose of the Study:

  • To review recent discoveries on the role of lncRNAs in regulating alternative splicing.
  • To explore the mechanisms by which lncRNAs influence splice isoform expression.

Main Methods:

  • Literature review of recent research on lncRNA-mediated alternative splicing regulation.
  • Analysis of identified mechanisms linking lncRNAs to splice isoform modulation.

Main Results:

  • lncRNAs play a significant role in the regulation of alternative splicing.
  • Multiple mechanisms exist through which lncRNAs shape the expression of diverse splice isoforms.
  • The discovery and characterization of lncRNAs are rapidly expanding.

Conclusions:

  • lncRNAs are crucial regulators of alternative splicing.
  • The impact of lncRNAs on alternative splicing is expected to increase with ongoing research.
  • Understanding lncRNA-splicing interactions is vital for comprehending cellular processes and disease mechanisms.