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Updated: Nov 10, 2025

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

An Improved Transwell Design for Microelectrode Ion-Flux Measurements.

Boris Buchroithner¹, Pavel Spurný², Sandra Mayr¹

¹School of Medical Engineering and Applied Social Sciences, University of Applied Sciences Upper Austria, Garnisonstr. 21, 4020 Linz, Austria.

|April 3, 2021

Summary

A novel transwell system enables microelectrode ion flux estimation (MIFE) on cell monolayers. This method supports cell growth and physiological responses for advanced cellular transport studies.

Keywords:

BisSR HUVECs MIFE biocompatible polymers ion flux multiphoton lithography

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Last Updated: Nov 10, 2025

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Area of Science:

Cellular Biology
Biophysics
Electrophysiology

Background:

Microelectrode ion flux estimation (MIFE) is a key technique for studying cellular membrane transport.
Traditional MIFE methods are typically performed in tissue culture dishes or directly on plant/animal tissues.
Limitations exist in applying MIFE to cell monolayers in a way that allows for controlled microenvironment manipulation and coculture.

Purpose of the Study:

To develop and validate a transwell system for performing MIFE measurements on cell monolayers.
To enable direct cell-to-cell contact coculture within the MIFE measurement setup.
To optimize the use of 3D printed polymer structures for cell support in transwell MIFE.

Main Methods:

Development of a modified transwell insert with a measurement window for MIFE.
Utilizing three-dimensional multiphoton lithography (MPL) to fabricate 3D polymer grid structures for cell support.
Optimization of the polymer grid constant for transwell MIFE applications.
Culturing and assessing human umbilical vein endothelial cells (HUVECs) on the 3D polymer structures.

Main Results:

Successful implementation of a transwell system for MIFE measurements on cell monolayers.
Demonstration that MPL-fabricated 3D polymer grids provide effective support for cell growth within the transwell.
Confirmation that HUVECs maintain their physiological functions and viability on the polymer structures.
Establishment of optimal polymer grid parameters for transwell MIFE.

Conclusions:

The developed transwell system is a viable platform for non-invasive electrophysiological studies of cell monolayers using MIFE.
This system facilitates controlled microenvironment studies and coculture applications for cellular transport research.
The integration of MPL-generated scaffolds enhances cell support and physiological relevance in transwell-based MIFE assays.