Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Digital Tattoos</i> in Infectious Diseases Management.

Open forum infectious diseases·2026
Same author

CPPD-Induced Iliopsoas Bursitis Mimicking Pyomyositis.

Clinical case reports·2026
Same author

Racial/Ethnic Disparities in Neoplasm-Related Mortality and the Social Determinants of Health.

Cancers·2026
Same author

Draft genome sequence of <i>bla</i><sub>IMP-1</sub>-carrying <i>Phytobacter ursingii</i> OUH-01, isolated from the feces of an acute lymphoblastic leukemia patient.

Microbiology resource announcements·2026
Same author

Phase Difference between End-Tidal CO<sub>2</sub> and Ventilation in Heart Failure with Oscillatory Breathing.

Cardiology·2026
Same author

Functional trihydroxyl germanium moiety in THGP confers novel enzyme activity inhibition abilities while also attenuating intracellular effects against multidrug-resistant Acinetobacter baumannii.

Metallomics : integrated biometal science·2026

Related Experiment Video

Updated: Nov 10, 2025

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

12.1K

Multiplex Real-Time PCR Assay for Six Major Carbapenemase Genes.

Nori Yoshioka1,2, Hideharu Hagiya1,3, Matsuo Deguchi1,2

  • 1Division of Infection Control and Prevention, Osaka University Hospital, 2-15 Yamadaoka, Suita, Osaka 565-0871, Japan.

Pathogens (Basel, Switzerland)
|April 3, 2021
PubMed
Summary
This summary is machine-generated.

A new multiplex real-time PCR assay rapidly detects diverse carbapenemase-producing Enterobacteriaceae (CPE). This cost-effective method aids early diagnosis of CPE infections, crucial for public health due to rising antimicrobial resistance.

Keywords:
carbapenem-resistant Enterobacteriaceaecarbapenemase-producing Enterobacteriaceaeinfection controlmultiplex detection assay

More Related Videos

Multiplex PCR and Reverse Line Blot Hybridization Assay mPCR/RLB
10:15

Multiplex PCR and Reverse Line Blot Hybridization Assay mPCR/RLB

Published on: August 6, 2011

44.0K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

753

Related Experiment Videos

Last Updated: Nov 10, 2025

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

12.1K
Multiplex PCR and Reverse Line Blot Hybridization Assay mPCR/RLB
10:15

Multiplex PCR and Reverse Line Blot Hybridization Assay mPCR/RLB

Published on: August 6, 2011

44.0K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

753

Area of Science:

  • Clinical Microbiology
  • Molecular Diagnostics
  • Infectious Diseases

Background:

  • Carbapenemase-producing Enterobacteriaceae (CPE) pose a significant global public health threat.
  • The diversity of carbapenemase genes necessitates effective detection methods.
  • Existing diagnostic tools may not be universally accessible or cost-effective.

Purpose of the Study:

  • To develop a multiplex real-time PCR assay for rapid and accurate detection of CPE.
  • To target common carbapenemase genes including NDM, KPC, IMP, GES, OXA-48, and VIM.
  • To utilize readily available clinical laboratory equipment for broad applicability.

Main Methods:

  • Development of a multiplex real-time PCR assay on the COBAS® z480 platform.
  • Design of two assay sets targeting combinations of specific carbapenemase genes (Set 1: blaNDM, blaKPC, blaIMP; Set 2: blaGES, blaOXA-48, blaVIM).
  • Validation using DNA standards, reference strains, and clinical isolates, including those with multiple carbapenemase genes.

Main Results:

  • The assay demonstrated high accuracy, with correlation coefficients >0.99 for all genotypes.
  • Accurate differentiation of strains harboring specific carbapenemase genes without cross-reactivity.
  • Successful detection of multiple carbapenemase genes in clinical isolates without false positives.

Conclusions:

  • The developed multiplex real-time PCR assay is accurate, robust, and rapid (1 hour).
  • It offers a cost-effective and simple solution for detecting diverse CPE.
  • This assay has the potential to significantly improve early diagnosis and management of CPE infections.