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Synthesis of hexanucleotide analogues containing diisopropylsilyl internucleotide linkages
1Department of Chemistry, McGill University, Montreal, Quebec, Canada.
Nucleic Acids Research
|May 25, 1988
Summary
Researchers synthesized silyl-linked hexanucleotide analogues. The thymidine analogue closely resembles its phosphodiester counterpart, based on hypochromicity and circular dichroism measurements.
Area of Science:
- Oligonucleotide chemistry
- Supramolecular chemistry
Background:
- Phosphodiester linkages are the natural backbone of DNA and RNA.
- Modifications to the DNA backbone are explored for various applications.
Purpose of the Study:
- To synthesize novel silyl-linked hexanucleotide analogues.
- To characterize the structural and binding properties of these analogues.
Main Methods:
- Chemical synthesis of silyl-linked hexanucleotides.
- Spectroscopic analysis including hypochromicity and circular dichroism (CD) measurements.
Main Results:
- Successful synthesis of two silyl-linked hexanucleotide analogues.
- Hypochromicity and CD data show the thymidine analogue mimics the structure of natural DNA.
Conclusions:
- Silyl-linked analogues are viable alternatives to phosphodiester backbones.
- The thymidine analogue exhibits similar duplex stability and conformation to natural DNA.