Related Experiment Video
Updated: Nov 10, 2025
07:16
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
2.0K
Stoichiometric Thiol Redox Proteomics for Quantifying Cellular Responses to Perturbations
Nicholas J Day1, Matthew J Gaffrey1, Wei-Jun Qian1
1Pacific Northwest National Laboratory, Biological Sciences Division, Richland, WA 99352, USA.
Antioxidants (Basel, Switzerland)
|April 3, 2021
Summary
Quantitative redox proteomics uses mass spectrometry to measure oxidative modifications on protein cysteine residues. This technique accurately quantifies thiol modifications, advancing redox biology research.
Area of Science:
- Biochemistry
- Proteomics
- Redox Biology
Background:
- Post-translational modifications regulate protein function and cellular pathways.
- Cysteine residues are sensitive to oxidative environments, undergoing thiol modifications.
- Mass spectrometry is key for identifying protein thiol modifications.
Purpose of the Study:
- To review advancements in mass spectrometry-based redox proteomics.
- To highlight methods for quantifying thiol modifications on cysteine residues.
- To emphasize the importance of quantitative redox proteomics in redox biology.
Main Methods:
- Mass spectrometry-based redox proteomics.
- Quantitative analysis of reversible oxidative modifications.
- Absolute quantitation of protein thiol modifications.
Main Results:
- Mass spectrometry enables accurate quantification of cysteine thiol modifications.
- Methodological advancements allow for absolute quantitation of these modifications.
- Recent studies demonstrate the application of these quantitative approaches.
Conclusions:
- Quantitative redox proteomics is crucial for understanding redox-sensitive pathways.
- Accurate measurements of thiol modifications provide insights into protein function.
- This approach significantly contributes to the field of redox biology.
Related Concept Videos
01:21Redox Titration: Overview
3.8K
Redox titration is a chemical analysis technique used to determine the concentration of an unknown substance by measuring the electron transfer in a redox (reduction-oxidation) reaction. The process involves gradually adding a titrant with a known concentration of an oxidizing or reducing agent, to the analyte, the solution with an unknown concentration, until reaching the endpoint, which indicates the completion of the reaction between the two substances. Ensuring the analyte is in a single...
3.8K
01:24Redox Reactions
57.2K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
57.2K
01:27Redox Reactions
431
Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
431