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LeafJ: An ImageJ Plugin for Semi-automated Leaf Shape Measurement
Published on: January 21, 2013
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Automatic Measurement of Morphological Traits of Typical Leaf Samples
Xia Huang1,2, Shunyi Zheng1,2, Li Gui1,2
1School of Remote Sensing and Information Engineering, Wuhan University, Wuhan 430079, China.
Sensors (Basel, Switzerland)
|April 3, 2021
Summary
A new method uses a handheld 3D laser scanner for automatic plant measurement, accurately selecting leaf samples and estimating traits even with canopy occlusion. This technology offers efficient, real-time plant phenotyping potential.
Area of Science:
- Agricultural Engineering
- Computer Vision
- Plant Science
Background:
- Automatic plant measurement is crucial for agricultural research and development.
- Existing methods face challenges with occluded plants and require manual intervention.
- Accurate plant phenotyping is essential for high-throughput screening and crop improvement.
Purpose of the Study:
- To propose a novel method for automatic plant measurement using a handheld 3D laser scanner.
- To enable automatic selection of typical leaf samples and estimation of morphological traits from occluded plants.
- To assess the accuracy, efficiency, and robustness of the proposed method.
Main Methods:
- Data acquisition using a handheld 3D laser scanner to create high-precision 3D plant models.
- Data processing involving multi-level region growing segmentation and two leaf shape models for leaf extraction.
- Estimation of four scale-related and six scale-invariant morphological traits.
Main Results:
- Successfully scanned 94.02% of typical leaf samples across different canopy occlusions.
- Achieved 87.61% accuracy in automatic extraction of typical leaf samples.
- Demonstrated high correlation (EF > 0.8919 for scale-related, EF > 0.7434 for scale-invariant traits) with manual measurements.
- Completed plant measurement in an average of 196.37 seconds.
Conclusions:
- The proposed method provides a robust and efficient solution for automatic plant measurement.
- It shows significant potential for real-time plant measurement and high-throughput phenotyping, even with canopy occlusion.
- The low time cost and high accuracy make it suitable for practical applications in plant science.

