The Loop-Mediated Isothermal Amplification Technique in Periodontal Diagnostics: A Systematic Review

Area of Science:

• Periodontology
• Molecular Diagnostics
• Microbiology

Background:

• Periodontal disease progression is influenced by dysbiotic subgingival microflora.
• Accurate and rapid identification of periodontal pathogens is crucial for effective treatment and risk assessment.

Purpose of the Study:

• To present loop-mediated isothermal amplification (LAMP) as an innovative technique for rapid bacterial panel identification in periodontal diseases.
• To highlight the advantages of LAMP over existing molecular and microbiological methods.

Main Methods:

• Systematic review of loop-mediated isothermal amplification (LAMP) for bacterial identification in periodontal disease.
• Comparison of LAMP with polymerase chain reaction (PCR/qPCR) and classical microbiological seeding techniques.

Main Results:

• LAMP allows simultaneous identification of multiple periodontal pathogens with high sensitivity.
• LAMP significantly reduces identification time from days to minutes.
• LAMP requires minimal hardware, making it suitable for outpatient settings.

Conclusions:

• LAMP is a rapid, sensitive, and potentially point-of-care method for diagnosing periodontal disease.
• This technique facilitates immediate periodontal status and complication risk assessment in clinical practice.