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Updated: Nov 10, 2025

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

Type 1 conventional dendritic cell fate and function are controlled by DC-SCRIPT.

Shengbo Zhang^1,2, Hannah D Coughlan^1,2, Mitra Ashayeripanah³

¹Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia.

Science Immunology

|April 3, 2021

Summary

This summary is machine-generated.

The transcription factor DC-SCRIPT is essential for the development and function of conventional dendritic cells (cDCs), specifically cDC1s. Its absence impairs immune responses by affecting antigen presentation and IL-12p40 secretion.

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Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

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Published on: May 18, 2018

Area of Science:

Immunology
Cell Biology
Genetics

Background:

Dendritic cells (DCs) are crucial for adaptive immunity.
The genetic regulation of DC functional diversification remains incompletely understood.
Conventional DCs (cDCs) and plasmacytoid DCs (pDCs) represent distinct DC lineages.

Purpose of the Study:

To investigate the role of the transcription factor DC-SCRIPT in DC development and function.
To elucidate the genetic basis of cDC1 identity and function.
To understand the regulatory network governing cDC1 lineage commitment.

Main Methods:

Analysis of DC-SCRIPT-deficient mice.
Flow cytometry to assess DC populations.
Genome-wide mapping of DC-SCRIPT binding sites (ChIP-seq).
Gene expression analysis (RNA-seq).

Main Results:

DC-SCRIPT deficiency led to impaired development of interferon regulatory factor 8 (IRF8)-dependent cDC1s.
Residual cDC1s in DC-SCRIPT-deficient mice showed reduced antigen-presenting capacity and IL-12p40 secretion.
DC-SCRIPT directly regulates key cDC1 signature genes, including Irf8, maintaining cDC1 identity.

Conclusions:

DC-SCRIPT is a critical transcription factor for cDC1 development and function.
DC-SCRIPT plays a vital role in the gene regulatory program that defines cDC1 identity.
Understanding DC-SCRIPT's function provides insights into immune response regulation.